Table 3 List of strains
From: LysX2 is a Mycobacterium tuberculosis membrane protein with an extracytoplasmic MprF-like domain
Strains | Relevant genotype and/or characteristics | Source or reference |
|---|---|---|
Escherichia coli | ||
DH5α | F- end A1 glnV44 thi- 1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK−mK+), λ− | Laboratory stock |
JM109 | endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F’ traD36 proAB+ lacIq lacZΔM15] hsdR17(rK−mK+) | Laboratory stock |
M. smegmatis | ||
mc2155 | parental strain | Laboratory stock |
MS321 | mc2155 with pROL-HYG | This work |
MS322 | mc2155 with pROL-HYG::Mt_lysX2 | This work |