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Table 3 Reagents and used volume for quantitative assessment of IL-1β and TNFα genes

From: Effects of probiotics on Zebrafish model infected with Aeromonas hydrophila: spatial distribution, antimicrobial, and histopathological investigation

Reagents Volume (Total Volume 10 µl)
Real Q Plus 2 × Master Mix Green
(HIGH ROX)
Including: KCl, MgCl2, dNTP,
taq DNA polymerase
5 µl
cDNA 2 µl
Forward Primers 0.5 µl
Reverse Primers 0.5 µl
DEPC water 2 µl