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Table 3 Reagents and used volume for quantitative assessment of IL-1β and TNFα genes

From: Effects of probiotics on Zebrafish model infected with Aeromonas hydrophila: spatial distribution, antimicrobial, and histopathological investigation


Volume (Total Volume 10 µl)

Real Q Plus 2 × Master Mix Green


Including: KCl, MgCl2, dNTP,

taq DNA polymerase

5 µl


2 µl

Forward Primers

0.5 µl

Reverse Primers

0.5 µl

DEPC water

2 µl