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Table 1 The primers used in the current report

From: Reliable detection of Burkholderia pseudomallei using multiple cross displacement amplification label-based biosensor

Primers a Sequences and modifications b Length c Genes
F1 5’-TCCCCCAATGCGCCAC-3’ 16 nt BPSS1406
F2 5’-CGCCTGCAACCACAGC-3’ 16 nt
CP1 5’-AACGGGAATCGGCCCGATGTTCTCGAATTGTCGTTGGAC-3’ 39 mer
CP2 5’-TTGCTTGCGGCCGAGATCGACGGAAAGAGCAGATTGAAG-3’ 39 mer
C1 5’-AACGGGAATCGGCCCGATG-3’ 19 nt
C1* 5’-FITC-AACGGGAATCGGCCCGATG-3’ 19 nt
C2 5’-TTGCTTGCGGCCGAGATCG-3’ 19 nt
D1 5’-CGTCGCTGGATGAGAA-3’ 16 nt
D2 5’-CCTGCCTGGCGAGAC-3’ 15 nt
D2* 5’-Biotin-CCTGCCTGGCGAGAC-3’ 15 nt
R1 5’-CCTGGCGAGCGTACT-3’ 15 nt
R2 5’-AGTCTCCTGCTCCCACA-3’ 17 nt
  1. aC1*, 5’-labeled with FITC when used in B. pseudomallei-MCDA-NB assay; D2*, 5’-labeled with Biotin when used in B. pseudomallei-MCDA-NB assay
  2. bFITC fluorescein isothiocyanate
  3. cmer monomeric unit, nt nucleotide