Table 5 The information of primers used in this study

cnf

AAGATGGAGTTTCCTATGCAGGAG

TGGAGTTTCCTATGCAGGAG

498

Cytotoxic necrotizing factor

Johnson & Stell. (2000)

feoB

AATTGGCGTGCATGAAGATAACTG

AGCTGGCGACCTGATAGAACAATG

470

Ferrous iron transporter

Yamamoto et al. (1995)

irp2

AAGGATTCGCTGTTACCGGAC

AACTCCTGATACAGGTGGC

413

Yersiniatbactin biosynthesis

Ewers et al. (2005)

iroD

AAGTCAAAGCAGGGGTTGCCCG

GACGCCGACATTAAGACGCAG

665

Catecholate siderophore receptor

Johnson. (2000)

ibeA

AGGCAGGTGTGCGCCGCGTAC

TGGTGCTCCGGCCAACCATGC

170

Invasion of brain endothelium

Johnson & Stell. (2000)

fimH

TGCAGAACGGATAAGCCGTGG

GCAGTCACCTGCCCTCCGGTA

508

Type 1 fimbriae

Johnson & Stell. (2000)

LT

TATCCTCTCTATATGCACAG

CTGTAGTGGAAGCTGTTATA

480

Enterotoxins

Osman et al. (2012)

kpsM

CATCATCAAATGGCAAGA

AAGCAGTATCGGCAGGAC

394

capsular polysaccharide synthesis

AF007777.1

ompT

TCATCCCGGAAGCCTCCCTCACTACTAT

TAGCGTTTGCTGCACTGGCTTCTGATAC

496

Outer member protein

MG149556.1

iutA

GGCTGGACATCATGGGAACTGG

CGTCGGGAACGGGTAGAATCG

302

Iron acquisition system

JX466848.1

iss

CAGCAACCCGAACCACTTGATG

AGCATTGCCAGAGCGGCAGAA

323

serum resistance

AF042279.1

hlyA

AACAAGGATAAGCACTGTTCTGGCT

ACCATATAAGCGGTCATTCCCGTCA

1177

α-Hemolysin

Yamamoto et al. (1995)

ompA

AAATACGGTAGAGTCAGGTGG

CGTTCACGCTTAATAAATGG

330

Outer membrane protein

FJ158545.1

TEM

CATTTCCGTGTCGCCCTTATTC

CGTTCATCCATAGTTGCCTGAC

800

β-lactamases resistance

Yao F et al.(2007)

SHV

AGCCGCTTGAGCAAATTAAAC ATCCCGCAGATAAATCACCAC

713

OXA

GGCACCAGATTCAACTTTCAAG GACCCCAAGTTTCCTGTAAGTG

564

CTX-M

CGCTTTGCGATGTGCAG ACCGCGATATCGTTGGT

550

strA

GCCAAAGGTCGAGGTGTGG CCAGTTCTCTTCGGCGTTAG

515

Aminoglycosides resistance

van Overbeek et al. (2002)

strB

GACTCCTGCAATCGTCAAGG GCAATGCGTCTAGGATCGAG

560

aadA

CAGCGCAATGACATTCTTGC

GTCGGCAGCGACATCCTTCG

295

gyrA

CGATGTCGGTCATTGTTG

CTTCCGTCAGGTTGTGC

496

Fluoroquinolones/Quinolones resistance

MF374502.1