Fig. 2

Evolutionary history of Stenotrophomonas strains based on concatenated alleles from the S. maltophilia MLST scheme (pubmlst.org). The figure combines data from Ochoa-Sánchez and Vinuesa and our study (Ochoa-Sánchez and Vinuesa, 2017). Lineages #1-#10 were defined by Hauben et al. (Hauben et al., 1999). Lineages (A) to (F) were defined by Kaiser et al. (Kaiser et al., 2009) and Ochoa-Sanchez et al. (Ochoa-Sanchez 2017) expanded this further. The putative species A-N from the ANIb analysis are indicated by ps and the letter. The evolutionary history was inferred using the Neighbor-Joining method (Saitou, et al., 1987). The optimal tree with the sum of branch length = 1.82770971 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches (Felsenstein, 1985). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The analysis involved 190 amino acid sequences. All ambiguous positions were removed for each sequence pair. There were a total of 3710 positions in the final dataset. Evolutionary analyses were conducted in MEGA X (Kumar et al., 2018). The left column denotes the isolates and strain identification. The right column the grouping. Type strains are indicated by (T) behind the name.