Emergence of amoxicillin resistance and identification of novel mutations of the pbp1A gene in Helicobacter pylori in Vietnam

Table 4 Overview of amino acid differences in PBP1A proteins occurring in clinical samples with resistance and sensitivity to amoxicillin

Sequence groups	E-test for Amoxicillin^b	Clinical samples	Amino acid position^a
Sequence groups	E-test for Amoxicillin^b	Clinical samples	366	414	Ins1^c	469	473	479	Ins2^d
Group (a)	Sensitive (0.047–0.125)	A283, A407, A105, A186, A146, A207, A271, A142, A481	Leu	Ser Arg	Glu	Val	Val	Glu	Gly Ser
Group (a)	Resistant (0.190–1.000)	A132, A009, A234, A482, A102, A100	Leu	Ser Arg	Lys Glu -	Val Met	Val	Glu Asp	Gly Ser Ala
Group (b)	Sensitive (0.032–0.125)	A119, A312, A404, A334	Leu	Ser Arg	Glu	Val	Val	Glu	Gly Ser
Group (b)	Resistant (0.190–2.000)	A147, A124, A277, A353, A162, A298, A255, A005, A141, A182	Leu	Arg	Lys Glu -	Val Met	Val	Glu Asp	Gly Ser Ala -
Group (c)	Sensitive (0.016–0.125)	A076, A101, A190, A291, A313, A336, A340, A131, A165, A081, A335, A027	Phe Leu	Ser	–	Val	Phe Val	Glu	Gly -
26,695	Sensitive	–	Phe	Ser	–	Val	Phe	Asp	–
Hagenberg	Resistant	–	Phe	Arg	–	Val	Phe	Asp	–

^a Positions of amino acid differences are given to the relative start point of the pbp1A gene of H. pylori Hagenberg (AF479617); ^b mg/l; ^c Amino acid inserted between positions 464 and 465; ^d Amino acid inserted between positions 595 and 596

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