Fig. 1
Isolation and pathogenicity tests of Pseudomonas savastanoi strain MHT1. A and B. Representative leaf and stem of Actinidia chinensis plants with bacterial canker symptoms sampled from the Xiache kiwifruit plant base (115°04′N, 24°64′E), Heping County, Heyuan City, Guangdong Province, China. Morphology of Pseudomonas savastanoi MHT1 colonies. A single colony of the MH1 strain was streaked and cultivated on LB medium at 28 °C for 24 h. D and E. Symptoms on the leaves and tender buds of A. chinensis plants (HongYang kiwifruit in C; WuZhi kiwifruit in D) generated by P. savastanoi MHT1 strains 8 days post-inoculation. A. chinensis leaves were infiltrated with bacterial suspensions (108 CFU/mL in 1 mM MgCl2) and photographed 8 days later. CFU, colony-forming units. Negative control, leaves infiltrated with 10 mM MgCl2. F and G. Symptoms on the leaves of A. chinensis plants (HongYang kiwifruit) generated by P. savastanoi MHT1 strains 6 days post-infection. Abaxial side leaves of A. chinensis were sprayed with bacterial suspensions (108 CFU/mL in 1 mM MgCl2) and photographed 6 days later