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Table 2 Cytokinins (CKs) scanned for using liquid chromatography-positive electrospray ionization tandem mass spectrometry in Methylobacterium supernatants

From: A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones

Cytokinin (CK)

Labelled CK Standard

Nucleotides (mono- di- and triphosphates; CK-NTs)

 trans-Zeatin nucleotide (tZNT)


 cis-Zeatin nucleotide (cZNT)

 Dihydrozeatin nucleotide (DHZNT)

 Isopentenyladenine nucleotide (iPNT)


Ribosides (CK-RBs)

 trans-Zeatin riboside (tZR)


 cis-Zeatin riboside (cZR)

 Dihydrozeatin riboside (DHZR)


 Isopentenyladenosine (iPR)


Free Bases (CK-FBs)

 trans-Zeatin (tZ)


 cis-Zeatin (cZ)

 Dihydrozeatin (DHZ)


 Isopentenyladenine (iP)


Glucosides (CK-GLUCs)

 trans-Zeatin-O-glucoside (tZOG)


 cis-Zeatin-O-glucoside (cZOG)

 Dihydrozeatin-O-glucoside (DHZOG)


 trans-Zeatin-O-glucoside riboside (tZROG)


 cis-Zeatin-O-glucoside riboside (cZROG)

 Dihydrozeatin-O-glucoside riboside (DHZROG)


 trans-Zeatin-9-glucoside (tZ9G)


 cis-Zeatin-9-glucoside (cZ9G)

 Dihydrozeatin-9-glucoside (DHZ9G)


Methylthiols (2MeS-CKs)

 2-Methylthio-Zeatin (2MeSZ)a


 2-Methylthio-Zeatin riboside (2MeSZR)a


 2-Methylthio-N6-isopentenyladenine (2MeSiP)


 2-Methylthio-N6-isopentenyladenosine (2MeSiPR)


  1. aThe analytical procedure used in this study does not facilitate separation of 2MeStZ and 2MeStZR from their corresponding cis-isomers, 2MeScZ and 2MeScZR. Therefore, the levels of the methylthiolated Z-type CKs were reported as total 2MeSZ and total 2MeSZR