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Table 2 Cytokinins (CKs) scanned for using liquid chromatography-positive electrospray ionization tandem mass spectrometry in Methylobacterium supernatants

From: A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones

Cytokinin (CK) Labelled CK Standard
Nucleotides (mono- di- and triphosphates; CK-NTs)
 trans-Zeatin nucleotide (tZNT) [2H3]DHZRMP
 cis-Zeatin nucleotide (cZNT)
 Dihydrozeatin nucleotide (DHZNT)
 Isopentenyladenine nucleotide (iPNT) [2H6]iPRMP
Ribosides (CK-RBs)
 trans-Zeatin riboside (tZR) [2H5]ZR
 cis-Zeatin riboside (cZR)
 Dihydrozeatin riboside (DHZR) [2H3]DHZR
 Isopentenyladenosine (iPR) [2H6]iPR
Free Bases (CK-FBs)
 trans-Zeatin (tZ) [2H5]Z
 cis-Zeatin (cZ)
 Dihydrozeatin (DHZ) [2H3]DHZ
 Isopentenyladenine (iP) [2H6]iP
Glucosides (CK-GLUCs)
 trans-Zeatin-O-glucoside (tZOG) [2H5]ZOG
 cis-Zeatin-O-glucoside (cZOG)
 Dihydrozeatin-O-glucoside (DHZOG) [2H7]DHZOG
 trans-Zeatin-O-glucoside riboside (tZROG) [2H5]ZROG
 cis-Zeatin-O-glucoside riboside (cZROG)
 Dihydrozeatin-O-glucoside riboside (DHZROG) [2H7]DHZROG
 trans-Zeatin-9-glucoside (tZ9G) [2H5]Z9G
 cis-Zeatin-9-glucoside (cZ9G)
 Dihydrozeatin-9-glucoside (DHZ9G) [2H5]DHZ9G
Methylthiols (2MeS-CKs)
 2-Methylthio-Zeatin (2MeSZ)a [2H5]2MeStZ
 2-Methylthio-Zeatin riboside (2MeSZR)a [2H5]2MeStZR
 2-Methylthio-N6-isopentenyladenine (2MeSiP) [2H6]2MeSiP
 2-Methylthio-N6-isopentenyladenosine (2MeSiPR) [2H6]2MeSiPR
  1. aThe analytical procedure used in this study does not facilitate separation of 2MeStZ and 2MeStZR from their corresponding cis-isomers, 2MeScZ and 2MeScZR. Therefore, the levels of the methylthiolated Z-type CKs were reported as total 2MeSZ and total 2MeSZR