Osmotic stress induces long-term biofilm survival in Liberibacter crescens

BMC Microbiology

Table 1 The effect of heat, sucrose, and/or DMSO in the growth parameters of L. crescens

Condition			Growth rate constant, k	Mean generation	p value
Heat	Sucrose	DMSO	(generation/h)¹	time, g (h)²
26°C³	—	—	0.039 ± 0.007	25.8	NS⁴
28 °C	—	—	0.040 ± 0.009	25.1	NS
30 °C	—	—	0.024 ± 0.002	41.6	≤ 0.05
32 °C	—	—	0.021 ± 0.003	48.1	≤ 0.05
—	50 mM	—	0.035 ± 0.003	28.7	NS
—	100 mM	—	0.025 ± 0.001	40.1	≤ 0.05
—	150 mM	—	0.012 ± 0.001	83.6	≤ 0.05
—	200 mM	—	0.007 ± 0.002	142.7	NS
—	—	0.01%	0.034 ± 0.001	29.8	NS
—	—	0.05%	0.029 ± 0.002	34.1	≤ 0.05
—	—	0.20%	0.027 ± 0.003	37.8	≤ 0.05

¹Growth rate constant (k) was calculated from the plot of log₂OD₆₀₀ versus time
²The mean generation time (g) was calculated as 1/k
³26 °C was considered as control conditions
⁴NS—NS indicates values that did not reach statistical significance

ISSN: 1471-2180