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Table 1 Detected species and average relative abundance for the HT-qPCR and 16S rRNA gene amplicon sequencing (NGS) in 21 Raclette du Valais PDO cheese samples

From: High-throughput qPCR and 16S rRNA gene amplicon sequencing as complementary methods for the investigation of the cheese microbiota

 

qPCR

NGS

 

Count

Avg. abund. [%]

SD

Count

Avg. abund. [%]

SD

Lacticaseibacillus paracasei

21

8.26

10.59

21

6.34

9.10

Lactococcus lactis

21

32.79

18.17

21

37.18

20.40

Streptococcus thermophilus

20

42.03

25.51

21

42.24

28.20

Lactiplantibacillus plantarum

20

12.00

14.76

20

3.47

6.56

Loigolactobacillus coryniformis

19

0.60

0.95

19

0.42

0.62

Lentilactobacillus parabuchneri

18

4.96

9.28

20

2.68

6.27

Latilactobacillus curvatus

18

0.77

0.85

19

0.26

0.30

Lactobacillus helveticus

5

3.28

2.48

21

0.63

1.47

Leuconostoc mesenteroides

9

0.31

0.22

14

0.13

0.15

Lactobacillus delbrueckii

4

1.58

1.54

19

0.66

1.89

Levilactobacillus brevis

7

0.47

0.61

16

0.26

0.55

Latilactobacillus sakei

10

0.24

0.25

12

0.10

0.12

Pediococcus pentosaceus

13

0.41

0.43

9

0.03

0.03

Lactiplantibacillus pentosus

0

  

20

5.21

7.91

Lacticaseibacillus rhamnosus

5

0.24

0.36

13

0.05

0.14

Enterococcus gilvus

0

  

15

0.03

0.05

Lactiplantibacillus paraplantarum

13

0.38

0.42

0

  

Lentilactobacillus sunkii

0

  

10

2.23

5.85

Streptococcus uberis

0

  

10

0.01

0.01

Latilactobacillus fuchuensis

0

  

9

0.02

0.02

Enterococcus faecalis

1

0.11

 

7

0.00

0.00

Paucilactobacillus nenjiangensis

0

  

8

0.14

0.20

Other species

0

  

20

0.05

0.06

  1. The relative abundance of the 21 species detected in more than 30% of the samples by NGS and Lactiplantibacillus paraplantarum exclusively detected by HT-qPCR are depicted with the species name; the other 26 taxa were classified as other species