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Fig. 1 | BMC Microbiology

Fig. 1

From: Sediment-associated microbial community profiling: sample pre-processing through sequential membrane filtration for 16S rRNA amplicon sequencing

Fig. 1

Schematic overview of the experimental procedure of the sediment-associated microbial community profiling employed in this study. A Collection of sediment samples. B Sequential membrane filtration from 10, 5 to 0.22 μm pore size filters as pre-processing step. C DNA extraction following the protocol of Zhou et al. (1996) (as employed in Solomon et al., 2016) with some modifications. D One-step PCR amplification of the 16S rRNA V4 hypervariable region. E Sequencing through the Illumina MiSeq Platform. F Bioinformatics and statistical data analysis were done in R (R Core Team, 2019)

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