Fig. 1
Validation of the correlation ship between YycG’ recombinant protein kinase and ATP consumption in vitro. Luminescence had a direct correlation with the consumption of ATP. A Data showed that a positive relationship between the RLU and the consumption of ATP with variant concentrations from 0 to 12.5 μM measured with the Kinase-Glo® Assay (R2 = 0.9671). B 3 μg of YycG’ recombinant protein was added into reaction system containing variant ATP concentrations (1.56 ~ 50 μM), systems without YycG’ present were used as control. C Variant amount of YycG’ proteins (0.5 ~ 32 μg) were added into the systems with 4 μM ATP. Data (means ± SDs) were performed in triplicate independently