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Table 2 Effect of enzymes, solvents and heat on the anti-Listeria activity

From: An antimicrobial peptide specifically active against Listeria monocytogenes is secreted by Bacillus pumilus SF214

Treatment

Activity on plate assaya

None

10

Trypsinb

0

Proteinase Kb

0

DNaseb

10

Ribonuclease Ab

10

Acetonec

10

Ethyl alcoholc

10

Chloroformc

10

Toluenec

10

15 min 60 °C

10

15 min 80 °C

10

15 min 100 °C

8

  1. a Diameter (mm) of the inhibition halo in plate assay
  2. b Enzyme concentration was 100 µg/ml
  3. c A 10% (v/v) concentration was used
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BMC Microbiology

ISSN: 1471-2180

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