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Table 1 Primers designed in this study for detection of MEL biosynthetic gene cluster

From: Detection and diversity of the mannosylerythritol lipid (MEL) gene cluster and lipase A and B genes of Moesziomyces antarcticus isolated from terrestrial sites chronically contaminated with crude oil in Trinidad

Gene target

Primer orientation

Primer Sequence (5′-3′)

Tma/°C

Amplicon size/bp

PaEmt1

Forward Primer

TGTCTGCGCTCGaaAGTaaG

62.194

848

Reverse Primer

GGAGAGCATaaACTGCGAGTAG

62.194

 

PaMac1

Forward Primer

GCATCTCGGAGCTGTACaaT

61.919

996

Reverse Primer

CGTGGaaCTTGGCATCaaAC

61.872

 

PaMac2

Forward Primer

ACTTGCCCTTTGGTCTGTT

61.999

685

Reverse Primer

ATTATCCGCCGCCTTGATT

61.986

 

PaMmf1

Forward Primer

GCTGATGCTGATTGCCTTTC

61.803

994

Reverse Primer

CCATCCGAGGaaGATGAGATTT

61.823

 

PaLipA

Forward Primer

GCGCTCaaGaaCTACCAGaa

62.016

666

Reverse Primer

GTCGaaGGCTTGCTTGATaaAC

61.941

 

PaLipB

Forward Primer

AGCCACTCCTTTGGTGaaG

61.847

724

Reverse Primer

GTaaGAGaaCTGCGAGGTGAG

61.804

 
  1. a Tm: primer annealing temperature