The lolB gene in Xanthomonas campestris pv. campestris is required for bacterial attachment, stress tolerance, and virulence

Table 4 Comparison of expression of putative lipoprotein encoding genes in the wild type XC17 and the lolB mutant CL17 by RT-qPCR

Gene ID ^a	Description	Predicted lipoprotein signal ^b	Fold change (CL17/XC17) ^c	p value
XC_0253	Dipeptidyl anmnopeptidase	MQRLLLASSLLLALSACSDKS	3.1015 ± 0.1651	0.0361
XC_0677	Hypothetical protein	MKYLLSAALCVAALSGCTDRE	6.9535 ± 0.4812	0.0312
XC_0679	Methanol dehydrogenase	MHQSSCRSARGGVLLMLALSAVLAGCKKDT	5.3956 ± 0.3450	0.0348
XC_0707	Rare lipoprotein A	MNSITGPKWLIPMALMLGLAACSSAP	3.0953 ± 0.2584	0.0733
XC_1519	Alkaline phosphatase	MPMRYRLPALAALTTLCVAACASTA	1.7876 ± 0.0305	0.0177
XC_1584	Cyanoglobin	MMTRWLRYSLLCVLTLSACATTQ	3.0530 ± 0.3839	0.0615
XC_3831	Hypothetical protein	MKIHWAVLACATLALAACQRPQ	2.2654 ± 0.1078	0.0056
XC_4152	Cytochrome c biogenesis protein	MARRFPWLWLGLLAACILVA	3.4164 ± 0.3353	0.0581

^a: Gene ID is based on X. campestris pv. campestris strain 8004
^b: According to the DOLOP database. The predicted lipobox with invariant cysteine is bold and underlined
^c: The relative expression level of each test gene in XC17 and CL17 was normalized to its 16S rRNA content. Values shown are the average ± standard deviation (n = 3)

ISSN: 1471-2180