Strain or plasmid | Description | Reference or source |
---|---|---|
E. coli | ||
 ECOS™ 101 | endA1 recA1 relA1 gyrA96 hsdR17(rK−, mK+) phoA supE44 thi-1 Δ(lacZYA-argF) U169 ϕ80Δ(IacZ)M15 F− | Yeastern |
X. campestris pv. campestris | ||
 XC17 | Virulent wild type strain isolated in Taiwan, ApR | [22] |
 CL17 | XC17-derived mutant with EZ-Tn5 inserted in lolB gene, ApR, KmR | This study |
Plasmid | ||
 yT&A | PCR cloning vector, ApR | Yeastern |
 pTlolB | A 790 bp RCR amplified fragment from lolB (nucleotides –133 to + 657 relative to the translation start site) and cloned into yT&A | This study |
 pUC19G | GmR cartridge from pUCGM ligated with the blunt-ended AvaII-SspI large fragment from pUC19 | [23] |
 pUClolB | The 790 bp BamHI-EcoRI fragment of the pTlolB cloned into the BamHI and EcoRI sites of pUC19G | This study |
 pUClolBK | pUClolB derivative with KmR inserted in the internal region of lolB gene | This study |
 pRK415 | Broad-host-range vector, RK2 ori, TcR | [24] |
 pRKlolB | The 790 bp BamHI-EcoRI fragment of the pTlolB cloned into the BamHI and EcoRI sites of pRK415 | This study |
 pFY13–9 | Promoter-probing vector derived from pRK415, using lacZ as the reporter, TcR | [25] |
 pFYengA | The 159-bp fragment, –181/–23 relative to engA translation start site, cloned into the PstI/XbaI sites of pFY13-9 | This study |
 pFYmanA | The 360-bp fragment, –372/–13 relative to manA translation start site, cloned into the PstI/XbaI sites of pFY13-9 | This study |
 pFYprt1 | The 313-bp fragment, –392/–80 relative to prt1 translation start site, cloned into the PstI/XbaI sites of pFY13-9 | [26] |
 pFYclpP1 | The 375-bp fragment, –384/–10 relative to clpP translation start site, cloned into the XhoI/XbaI sites of pFY13-9 | [27] |
 pFYclpX | The 327-bp fragment, –336/–10 relative to clpX translation start site, cloned into the XhoI/XbaI sites of pFY13-9 | This study |