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Table 1 Bacterial strains and plasmids used in this study

From: The lolB gene in Xanthomonas campestris pv. campestris is required for bacterial attachment, stress tolerance, and virulence

Strain or plasmid

Description

Reference or source

E. coli

 ECOS™ 101

endA1 recA1 relA1 gyrA96 hsdR17(rK−, mK+) phoA supE44 thi-1 Δ(lacZYA-argF) U169 ϕ80Δ(IacZ)M15 F−

Yeastern

X. campestris pv. campestris

 XC17

Virulent wild type strain isolated in Taiwan, ApR

[22]

 CL17

XC17-derived mutant with EZ-Tn5 inserted in lolB gene, ApR, KmR

This study

Plasmid

 yT&A

PCR cloning vector, ApR

Yeastern

 pTlolB

A 790 bp RCR amplified fragment from lolB (nucleotides –133 to + 657 relative to the translation start site) and cloned into yT&A

This study

 pUC19G

GmR cartridge from pUCGM ligated with the blunt-ended AvaII-SspI large fragment from pUC19

[23]

 pUClolB

The 790 bp BamHI-EcoRI fragment of the pTlolB cloned into the BamHI and EcoRI sites of pUC19G

This study

 pUClolBK

pUClolB derivative with KmR inserted in the internal region of lolB gene

This study

 pRK415

Broad-host-range vector, RK2 ori, TcR

[24]

 pRKlolB

The 790 bp BamHI-EcoRI fragment of the pTlolB cloned into the BamHI and EcoRI sites of pRK415

This study

 pFY13–9

Promoter-probing vector derived from pRK415, using lacZ as the reporter, TcR

[25]

 pFYengA

The 159-bp fragment, –181/–23 relative to engA translation start site, cloned into the PstI/XbaI sites of pFY13-9

This study

 pFYmanA

The 360-bp fragment, –372/–13 relative to manA translation start site, cloned into the PstI/XbaI sites of pFY13-9

This study

 pFYprt1

The 313-bp fragment, –392/–80 relative to prt1 translation start site, cloned into the PstI/XbaI sites of pFY13-9

[26]

 pFYclpP1

The 375-bp fragment, –384/–10 relative to clpP translation start site, cloned into the XhoI/XbaI sites of pFY13-9

[27]

 pFYclpX

The 327-bp fragment, –336/–10 relative to clpX translation start site, cloned into the XhoI/XbaI sites of pFY13-9

This study

  1. ApR, ampicillin-resistant; GmR, gentamycin-resistant; KmR, kanamycin-resistant; TcR, tetracycline-resistant