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Fig. 5 | BMC Microbiology

Fig. 5

From: The lolB gene in Xanthomonas campestris pv. campestris is required for bacterial attachment, stress tolerance, and virulence

Fig. 5

Effects of mutation of lolB on the expression of genes coding for extracellular enzymes or products associated with stress tolerance. (a) ß Galactosidase activities of XC17 (wild type, black bar) and CL17 (lolB mutant, gray bar) carrying pFYengA, pFYmanA, pFYprt1, pFYclpP1 and pFYclpX were determined. Tested strains were cultured in XOLN medium containing glycerol for 24 h. Significance was determined by the Student t test (* indicates significance at p < 0.05). (b) The expression level of extracellular enzyme genes (engA, manA, and prt1) and stress tolerance-related genes (clpP and clpX) in the wild-type strain XC17 and lolB mutant strain CL17 was examined by RT-qPCR. The relative expression level of each test gene in XC17 and CL17 was normalized to its 16S rRNA content. Values shown are the average ± standard deviation (n = 3)

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BMC Microbiology

ISSN: 1471-2180

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