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Table 2 Summary of in vitro monoculture experiments

From: Increased circulating butyrate and ursodeoxycholate during probiotic intervention in humans with type 2 diabetes

Measurement

Vendor

No. of Samples

Strains

Specimen

Untargeted

Metabolon

5 (pilot; 1 per strain)

AMUC, BINF, CBEI, CBUT, EHAL

supernatant, cell pellet

Bile acids

MS-Omics

30 per strain (NIC: 12, Inoc: 18)

AMUC, BINF, CBEI, CBUT, EHAL

supernatant, cell pellet

sulfonylurea sensitivity, growth dynamics

(in-house)

45 per strain per run (3 replicates, 5 titers, 3 SFUs); NIC: 12, DMSO-only: 12, Base medium: 12

AMUC, BINF, CBEI, CBUT, EHAL

N/A

  1. For the untargeted pilot, each strain received separate untargeted measurements of late-log phase supernatant and cell pellet. The uninoculated growth medium was separately measured as well
  2. NIC Not-inoculated control, Inoc Inoculated, SFU Sulfonylurea