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Fig. 1 | BMC Microbiology

Fig. 1

From: Proteome and secretome profiling of zinc availability in Cryptococcus neoformans identifies Wos2 as a subtle influencer of fungal virulence determinants

Fig. 1

Overview of experimental design and mass spectrometry workflow. Cell pellets and supernatant from C. neoformans cultured in minimal medium (MM) MM + Zn or MM-Zn were subjected to protein extraction (e.g., sonication and detergent) and enzyme digestion (e.g., trypsin/Lys-C) followed by purification (C18 STAGE-tips) and measurement on a quadrupole orbitrap mass spectrometer [37,38,39,40]. Data analysis and visualization performed with MaxQuant and Perseus. Experiment performed in biological quadruplicate. Figure generated with Biorender.com

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BMC Microbiology

ISSN: 1471-2180

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