Table 2 Test performance of multiplex polymerase chain reaction (M-PCR) in detection of Streptococcus pneumoniae and Haemophilus influenzae compared to culture method
| Â | Culture for Streptococcus pneumoniae | Culture for Haemophilus influenzae | ||||
|---|---|---|---|---|---|---|
Positive | Negative | Total | Positive | Negative | Total | |
PCR Positive | 15 (16.3%) | 18 (19.5%) | 33 (35.8%) | 7 (7.6%) | 4 (4.3%) | 11 (11.9%) |
PCR Negative | 0 (0.0%) | 59 (64.2%) | 59 (64.2%) | 0 (0.0%) | 81 (88.1%) | 81 (88.1%) |
Total | 15 (16.3%) | 77 (83.7%) | 92 (100%) | 7 (7.6%) | 85 (92.4%) | 92 (100%) |
Sensitivity (%) (95% CI) | 100.0% (89.4 to 100.0%) | 100.0% (71.5 to 100.0%) | ||||
Specificity (%) (95% CI) | 76.6% (65.6 to 85.5%) | 95.3% (88.4 to 98.7%) | ||||
Positive predictive value (%) (95% CI) | 64.7% (55.0 to 73.3%) | 73.3% (51.4 to 87.7%) | ||||
Negative predictive value (%) (95% CI) | 100.0% | 100.0% | ||||
Positive likelihood ratio (%) (95% CI) | 4.3% (2.9 to 6.4%) | 21.3% (8.2 to 55.3%) | ||||
Negative likelihood ratio (%) (95% CI) | 0.0% | 0.0% | ||||
Test accuracy (%) (95% CI) | 83.6% (75.4 to 90.0%) | 95.8% (89.7 to 98.9%) | ||||