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Table 4 Effect of the absence of ccpA gene on enzymes activity in S. bovis S1 at different pH (n = 3)

From: Regulation of CcpA on the growth and organic acid production characteristics of ruminal Streptococcus bovis at different pH

Items

Wild-type strain

ccpA-knockout strain

SEM1

P-value2

pH 6.5

pH 5.5

pH 6.5

pH 5.5

Strain

pH

Strain×pH

Lactate dehydrogenase (U/L)

20.46

23.66

17.85

19.65

0.659

< 0.001

< 0.001

0.137

α-amylase (U/L)

33.87

40.28

28.41

37.06

1.345

< 0.001

< 0.001

0.096

Acetokinase (U/L)

2.34c

2.43c

3.06a

2.71b

0.089

< 0.001

0.101

0.013

  1. 1 SEM, standard error of mean
  2. 2 Strain, strain effect; pH, pH effect; Strain × pH, the interaction between strain and pH
  3. a,b,c Means within a row with different superscripts differ significantly (P < 0.05)