Table 1 Primers used in this study
Primer names | Primer sequences (5′-3′) | Purpose of use | Product length (bp) | Reference or source |
|---|---|---|---|---|
ccpA up-F | TGTAAAACGACGGCCAGTGAATTCGTTCCAAGGTCAAACAAAAGTAGAG | Construction of ccpA-knockout mutant | 1053 | This study |
ccpA up-R | AAGCTGTCAAACATGAGAATTAGAGCTCTATTGGACTTCCTTTCTATTTG | |||
ccpA down-F | AGCTTTTGCTAAAGAAGAATTGGATCCTTTCCAAAAAGGATACTATGAC | Construction of ccpA-knockout mutant | 1101 | This study |
ccpA down-R | CAAGCTTGCATGCCTGCAGGTCGACGCAACTTTATCAATGCTACGAC | |||
erm-F | CAAATAGAAAGGAAGTCCAATAGAGCTCTAATTCTCATGTTTGACAGCTT | Construction of ccpA-knockout mutant | 1207 | This study |
erm-R | GTCATAGTATCCTTTTTGGAAAGGATCCAATTCTTCTTTAGCAAAAGCT | |||
ccpA 1-F | TGGTGAATCATTACTTGTAAGA | Verification of ccpA-knockout mutant | 426 | This study |
ccpA 1-R | GAGTTCTCTCGCTCACGCACAC | |||
ccpA 2-F | AAACCTTCTTTCTAATTACCCC | Verification of ccpA-knockout mutant | 1827 | This study |
ccpA 2-R | GGCATAAATCGGCTTGTCAACG | |||
16S-F | GAACACCGGTGGCGA | RT-qPCR | Asanuma et al. [23] | |
16S-R | CTCATCGTTTACGGCG | |||
pfl-F | GGTTACATCTACGACTACGA | RT-qPCR | 119 | Chen et al. [10] |
pfl-R | TGGCTACGAAGACGAGTA | |||
ldh-F | GGTTCTTCTTACGCATTCG | RT-qPCR | 190 | Chen et al. [10] |
ldh-R | TAACTACAAGGTCAGCATCT | |||
α-amy-F | TCAAGCACTGGAA TCAACTA | RT-qPCR | 109 | Chen et al. [10] |
α-amy-R | GCCGTAA TAA TCTCCGTAGA | |||
ack-F | TGGCCAGAAACAGTCGGAAA | RT-qPCR | 134 | This study |
ack-R | CTACCACACGGTGACCAACA |