Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum

Padilla-Gálvez, Natalia; Luengo-Uribe, Paola; Mancilla, Sandra; Maurin, Amandine; Torres, Claudia; Ruiz, Pamela; France, Andrés; Acuña, Ivette; Urrutia, Homero

doi:10.1186/s12866-021-02393-x

Table 1 Macro- and microscopic phenotype descriptions of putative endophyte actinobacteria of native Chilean Solanum tuberosum subsp. tuberosum with their taxonomic identity according to 16S ribosomal gene analysis

From: Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum

Isolate code	Origin of plant tissue	Culture media ^a			Aerial mycelia^b	Substrate mycelia^b	Soluble pigment^c	Filament diameter	Spore diameter	Spore disposition ^d	Identity by 16S ribosomal DNA partial gene	Closest taxa strain in NCBI	Nucleotide sequence	Similarity in Blast analysis	Accession in NCBI database
Isolate code	Origin of plant tissue	ISP2	ISP3	ISP4	Aerial mycelia^b	Substrate mycelia^b	Soluble pigment^c	Filament diameter	Spore diameter	Spore disposition ^d	Identity by 16S ribosomal DNA partial gene	Closest taxa strain in NCBI	Nucleotide sequence	Similarity in Blast analysis	Accession in NCBI database
H2TP199	Leaf	B	MB	MB	gray	orange	+	0.77 um	0.62 um	Rectiflexibiles	Streptomyces sp.	Streptomyces galilaeus strain P-YIM40 (GenBank: MT533915.1)	1051 bp	100%	KY242591
G1TP199	Root	MB	MB	B	gray	orange	+	< 0.5 um	< 0.5 um	ND ^d	Streptomyces sp.	Streptomyces sp. strain GF22 (GenBank: MN826279.1)	1036 bp	99.2%	KY242592
KR31	Stem	B	B	B	white	brown	+	0.72 um	< 0.5 um	Retinaculiaperti	Streptomyces sp.	Streptomyces sp. 7R006 (GeneBank: LC497916.1)	803 bp	98.9%	KY242593
DP143	Tubercle	MB	MB	MB	white/gray	brown	+	0.67 um	< 0.5 um	Retinaculiaperti	Streptomyces sp.	Streptomyces sp. YRA147 (GenBank: JX430828.1)	1104 bp	99.8%	KY242596
A2R31	Stem	MB	EX	B	gray	brown	+	0.77 um	0.89 um	Rectiflexibiles	Streptomyces achromogenes	Streptomyces achromogenes strain NBRC 12735	969 bp	100%	KY242590
CR34	Tubercle	MB	B	B	ND^e	cream	–	< 0.5 um	< 0.5 um	ND ^e	Nocardia sp.	Nocardia sp. FGT33 (GenBank: KU382293.1)	750 bp	99.9%	KY296348
HP171	Root	MB	MB	EX	white	orange	–	0.67 um	0.66 um	Rectiflexibiles	Streptomyces sp.	Streptomyces galilaeus strain PSWC-10 (GenBank: MT275786.1)	1027 bp	99.9%	KY242594
MP136	Leaf	MB	MB	MB	black	yellow	–	0.56 um	0.66 um	Spira	Streptomyces sp.	Streptomyces sp. CH9-7 (GenBank: LC489240.1)	1078 bp	99.8%	KY242595
TP199	Tubercle	EX	B	MB	gray	yellow	–	0.56 um	< 0.5 um	Straight to flexuous chains	Streptomyces sp.	Streptomyces sp. KK13-6 (GenBank: LC487830.1)	1147 bp	99.6%	KY228978
NP199	Tubercle	MB	MB	MB	gray	yellow	–	0.77 um	0.58 um	ND ^e	Streptomyces sp.	Streptomyces sp. HST09 (GenBank: KX130883.1)	1114 bp	99.3%	KY242597

^aCulture media according to [38], ISP2 (yeast extract and– malt extract agar), ISP3 (oatmeal agar) and ISP4 (inorganic salts and– starch agar). Furthermore, excellent (EX, 76 to 100%), very good (MB, 51 to 75%), good (B, 26 to 50%), poor (P, 1 to 25%), and negative (N, 0%)
^bDetermined on ISP2 medium according to [38]
^cNegative: -, Positive: +. Determined in ISP1 medium according to [38]
^dDescription according to [39] e ND Not determined

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ISSN: 1471-2180

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