Skip to main content

Table 4 Cycling conditions for ddPCR using P5 and P7 primer amplifying amplicons

From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

Cycling step

Temperature (°C)

Time

Ramp rate

Number of cycles

Enzyme activation

95

5 min

2 °C/s

1

Denaturation

95

30 s

40

Annealing

59

1 min

Extension

72

1 min

Signal stabilization

4

5 min

1

90

5 min

1

Hold

4

∞

 

1