From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples
Cycling step | Temperature (°C) | Time | Ramp rate | Number of cycles |
---|---|---|---|---|
Enzyme activation | 95 | 5 min | 2 °C/s | 1 |
Denaturation | 95 | 30 s | 40 | |
Annealing | 59 | 1 min | ||
Extension | 72 | 1 min | ||
Signal stabilization | 4 | 5 min | 1 | |
90 | 5 min | 1 | ||
Hold | 4 | ∞ |  | 1 |