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Table 4 Cycling conditions for ddPCR using P5 and P7 primer amplifying amplicons

From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

Cycling step Temperature (°C) Time Ramp rate Number of cycles
Enzyme activation 95 5 min 2 °C/s 1
Denaturation 95 30 s 40
Annealing 59 1 min
Extension 72 1 min
Signal stabilization 4 5 min 1
90 5 min 1
Hold 4   1