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Fig. 6 | BMC Microbiology

Fig. 6

From: Bacterial microcompartments for isethionate desulfonationĀ in the taurine-degrading human-gut bacterium Bilophila wadsworthia

Fig. 6

Specific enzyme activities detected in the different sucrose gradient fractions (B-E) in comparison to an image of a representative gradient (A).Ā Protein concentrations in the fractions 7-9 were typically very low and, thus, apparently high specific enzyme activities detected were considered unreliable. Specific protein activities are given as Units per mg of total protein. The figure shows means Ā± standard deviation of three technical replicates, representing one of two biological replicates. Asterisks denote statistical significance at a power of 0.05 (B) IslAB activity represented as sulfite (light gray bars) and acetaldehyde (dark gray bars) production in anoxic incubations with isethionate and S-adenosylmethionine. n.d.: not determined; n.m.: not measurable. CĀ Specific acetaldehyde dehydrogenase activity determined photometrically as rate of NADH formation from NAD+. Grey bars, total acetaldehyde dehydrogenase activity (measured in presence of free coenzyme A); red bars, acetylating acetaldehyde dehydrogenase activity, calculated by subtracting non-acetylating acetaldehyde dehydrogenase activity (measured in absence of free coenzyme A). DĀ Specific phosphotransacetylase activity measured as acetyl-CoA formation from acetyl phosphate in the presence of free coenzyme A. EĀ Specific acetate kinase activity measured as acetyl phosphate consumption in the presence of ADP

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