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Table 2 Growth tests were performed with media supplied with 5 % NaCl (w/v) in either media. Determination of the optimal NaCl was done in MRS pH 5.7 after 48 h of incubation. Results of the API-CHL 50 test in API-CHL medium supplied with 2 % NaCl. All strains were able to produce acid from D-glucose, D-fructose, D-mannose, N-acetyl-Glucosamine, arbutin, Esculin/iron citrate, salicin, D-cellobiose, D-maltose, sucrose, D-trehalose, D-gentobiose, D-turanose. None of the strains could produce acid from erythritol, L-xylose, D-adonitol, methyl-beta-D-xylopyranosid, L-sorbose, L-rhamnose, dulcitol, inositol, inulin, adonitol, glycogen, xylitol, D-lyxose, D-fucose, L-fucose, L-arabitol, potassium gluconate, potassium-2-ketogluconate, potassium-5-ketogluconate; +, positive reaction; w, weak reaction; -, negative reaction; ND, not determined

From: The diversity among the species Tetragenococcus halophilus including new isolates from a lupine seed fermentation

     T. halophilus
  T. osmophilus subsp. flandriensis subsp. halophilus  
  DSM 23765T DSM 23766T DSM 20339T DSM 20337 TMW 2.2254 TMW 2.2256 TMW 2.2257 TMW 2.2263 TMW 2.2264 TMW 2.2266
Growth at/in:           
30°C in TSA + + + + + + + + + +
30°C in MRS - - + + + + + + + +
Optimal NaCl (w/v) ND ND 9% 8% 9% 10% 12% 10% 10% 9%
Fermentation of carbohydrates:           
Glycerol - - - - - + + - - -
D-Arabinose - + - - - - - - - -
L-Arabinose - - + + - + - + - +
D-Ribose - + + + + + + + + +
D-Xylose - - - - - - - - - +
D-Galactose - w + + + + + + + +
D-Mannitol + - - + + + + + + +
D-Sorbitol - + - + - + + + - -
Methyl-alpha-D-Mannopyranosid + - - - - - + - - -
Methyl-alpha-D-Glucopyranosid + + + + + + + - - -
Amygdalin - + + + + + + + + +
D-Lactose - - - - - + - w - -
D-Melibiose - + - + + - - - + -
D-Melecitose - - + - - - + - - -
D-Raffinose - + - - - - - - - -
D-Tagatose - - + + + + + + - +
D-Arabitol + - + - + + + + + -