Table 1 Bacterial strains and plasmids
Strain or plasmid | Description | Reference or source |
|---|---|---|
Escherichia coli strains | ||
DH5α | Strain for cloning | [47] |
S17–1 | Strain for plasmid mobilization | [48] |
BL21(DE3) | Strain for protein expression | Novagen |
Chromobacterium violaceum strains | ||
ATCC 12472 | Wild-type strain (Sequenced genome) | [6] |
JF3905 | Wild-type strain with the CV_3905 gene deleted (∆osbR) | This work |
JF0210 | Wild-type strain with the CV_0210 gene deleted (∆ohrR) | [32] |
JF39050210 | Wild-type strain with the CV_3905 and CV_0210 genes deleted (ΔosbRΔohrR) | This work |
WT(pJN105) | Wild-type strain containing the vector pJN105 empty | This work |
WT(osbR) | Wild-type strain overexpressing osbR | This work |
∆osbR(pJN105) | Mutant osbR containing the vector pJN105 empty | This work |
∆osbR(osbR) | Mutant osbR strain overexpressing osbR | This work |
Plasmids | ||
pNPTS138 | Suicide vector containing oriT sacB; Kanamycin resistance | D. Alley |
pJN105 | Broad-host-range vector; Gentamycin resistance | [49] |
pET15b | His-tagged protein expression vector; Ampicillin resistance | Novagen |
pNPTS138 ∆osbR | In frame null deletion of osbR | This work |
pNPTS138 ∆ohrR | In frame null deletion of ohrR | [32] |
pJN105 osbR | Overexpression of osbR | This work |
pET15b osbR | Heterologous expression of the His-OsbR protein | This work |