|
Escherichia coli strains
|
|
DH5α
|
Strain for cloning
|
[47]
|
|
S17–1
|
Strain for plasmid mobilization
|
[48]
|
|
BL21(DE3)
|
Strain for protein expression
|
Novagen
|
|
Chromobacterium violaceum strains
|
|
ATCC 12472
|
Wild-type strain (Sequenced genome)
|
[6]
|
|
JF3905
|
Wild-type strain with the CV_3905 gene deleted (∆osbR)
|
This work
|
|
JF0210
|
Wild-type strain with the CV_0210 gene deleted (∆ohrR)
|
[32]
|
|
JF39050210
|
Wild-type strain with the CV_3905 and CV_0210 genes deleted (ΔosbRΔohrR)
|
This work
|
|
WT(pJN105)
|
Wild-type strain containing the vector pJN105 empty
|
This work
|
|
WT(osbR)
|
Wild-type strain overexpressing osbR
|
This work
|
|
∆osbR(pJN105)
|
Mutant osbR containing the vector pJN105 empty
|
This work
|
|
∆osbR(osbR)
|
Mutant osbR strain overexpressing osbR
|
This work
|
|
Plasmids
|
|
pNPTS138
|
Suicide vector containing oriT sacB; Kanamycin resistance
|
D. Alley
|
|
pJN105
|
Broad-host-range vector; Gentamycin resistance
|
[49]
|
|
pET15b
|
His-tagged protein expression vector; Ampicillin resistance
|
Novagen
|
|
pNPTS138 ∆osbR
|
In frame null deletion of osbR
|
This work
|
|
pNPTS138 ∆ohrR
|
In frame null deletion of ohrR
|
[32]
|
|
pJN105 osbR
|
Overexpression of osbR
|
This work
|
|
pET15b osbR
|
Heterologous expression of the His-OsbR protein
|
This work
|
