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Table 1 Bacterial strains and plasmids

From: The MarR family regulator OsbR controls oxidative stress response, anaerobic nitrate respiration, and biofilm formation in Chromobacterium violaceum

Strain or plasmid Description Reference or source
Escherichia coli strains
 DH5α Strain for cloning [47]
 S17–1 Strain for plasmid mobilization [48]
 BL21(DE3) Strain for protein expression Novagen
Chromobacterium violaceum strains
 ATCC 12472 Wild-type strain (Sequenced genome) [6]
 JF3905 Wild-type strain with the CV_3905 gene deleted (∆osbR) This work
 JF0210 Wild-type strain with the CV_0210 gene deleted (∆ohrR) [32]
 JF39050210 Wild-type strain with the CV_3905 and CV_0210 genes deleted (ΔosbRΔohrR) This work
 WT(pJN105) Wild-type strain containing the vector pJN105 empty This work
 WT(osbR) Wild-type strain overexpressing osbR This work
 ∆osbR(pJN105) Mutant osbR containing the vector pJN105 empty This work
 ∆osbR(osbR) Mutant osbR strain overexpressing osbR This work
Plasmids
 pNPTS138 Suicide vector containing oriT sacB; Kanamycin resistance D. Alley
 pJN105 Broad-host-range vector; Gentamycin resistance [49]
 pET15b His-tagged protein expression vector; Ampicillin resistance Novagen
 pNPTS138 ∆osbR In frame null deletion of osbR This work
 pNPTS138 ∆ohrR In frame null deletion of ohrR [32]
 pJN105 osbR Overexpression of osbR This work
 pET15b osbR Heterologous expression of the His-OsbR protein This work