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Fig. 5 | BMC Microbiology

Fig. 5

From: The MarR family regulator OsbR controls oxidative stress response, anaerobic nitrate respiration, and biofilm formation in Chromobacterium violaceum

Fig. 5

OsbR oxidation causes dimerization and inhibition of its DNA binding activity. a Formation of covalent OsbR dimers under oxidative stress in vitro. After 2 hours of exposure to oxidants or DTT, the His-OsbR protein was analyzed by non-reducing SDS-PAGE. NT refers to nontreated protein. Monomeric and dimeric forms are indicated by M and D, respectively. Asterisk indicates a third unknown multimeric form. b EMSA assay with oxidized OsbR. Prior to incubation with the DNA probes, His-OsbR was oxidized with 1 mM CHP for 1 hour (for the osbR probe, OsbR was oxidized with the indicated CHP concentrations)

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BMC Microbiology

ISSN: 1471-2180

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