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Fig. 6 | BMC Microbiology

Fig. 6

From: Multiple Cross Displacement Amplification Coupled with Lateral Flow Biosensor (MCDA-LFB) for rapid detection of Legionella pneumophila

Fig. 6

Optimized reaction time for MCDA assay. Four different reaction times (A, 25 min; B, 35 min; C, 45 min; D, 55 min) were tested and compared at optimal amplification temperature (65 °C). Biosensors 1, 2, 3 and 4 represent DNA template levels of 10 fg/μL (LoD level). The best sensitivity was seen when the amplification lasted for 35 min

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BMC Microbiology

ISSN: 1471-2180

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