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Fig. 5 | BMC Microbiology

Fig. 5

From: Multiple Cross Displacement Amplification Coupled with Lateral Flow Biosensor (MCDA-LFB) for rapid detection of Legionella pneumophila

Fig. 5

Sensitivity of the L. pneumophila-MCDA assay. Two detection techniques including (A) VDR analysis and (B) lateral flow biosensor were applied for MCDA products. Serial dilutions (10 ng/μL, 1 ng/μL, 100 pg/μL, 10 pg/μL, 1 pg/μL, 100 fg/μL, 10 fg/μL, 1 fg/μL) of L. pneumophila ATCC 33152 genomic DNA were used for sensitivity analysis. Tubes (A)/ Biosensor (B) 1–8 represented different concentrations of DNA, 10 ng/μL, 1 ng/μL, 100 pg/μL, 10 pg/μL, 1 pg/μL, 100 fg/μL, 10 fg/μL, 1 fg/μL, respectively; 9 represents a negative control (DW). The LoD of L. pneumophila-MCDA assay for mip gene detection was 10 fg per reaction

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BMC Microbiology

ISSN: 1471-2180

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