Fig. 4

Enrichment of Fusobacterium nucleatum in ESCC detected by FISH. Graph A: ESCC specimens stained with DAPI. Cell nuclei were stained in blue; B: ESCC specimens stained with both DAPI and a universal bacterial probe (EUB338). Bacterial conserved regions were stained in green; C: ESCC specimen stained with both DAPI and a Fusobacterium-specific probe (FUSO). The F. nucleatum-specific regions were stained in red; D: ESCC specimens triply stained with DAPI, FUSO and EUB338. Cell nuclei (in blue), bacterial conserved regions (in green) and F. nucleatum-specific regions (in red) were clearly visible (all × 400)