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Table 3 Mycobacterial strain identification using Hain Genotype line probe assay (LPA)

From: Bacteriological and molecular characterization of Mycobacterium bovis isolates from tuberculous lesions collected among slaughtered cattle, Northwest Ethiopia

Isolate nr. Line probe assay (LPA) result Mycobacterial identification
MTBc (TUB) rpoB WT rpoB MUT katG WT katG MUT inhA WT inhA MUT
H37Rv + + + + M. tuberculosis
Distilled water None
07 non-MTBc
12 + + + + MTBc
13 non-MTBc
16 + + + + MTBc
20 non-MTBc
21 + + + + MTBc
23 non-MTBc
36 + + + + MTBc
37 non-MTBc
38 non-MTBc
41 non-MTBc
43 non-MTBc
45 non-MTBc
48 + + + + MTBc
50 non-MTBc
54 non-MTBc
60 non-MTBc
61 non-MTBc
  1. The Hain Genotype LPA result from 18 culture-positive isolates obtained from LJ culture; five isolates (Isolate number: 12, 16, 21, 36, and 48) were confirmed as members of the M. tuberculosis complex (MTBc), while the remained 13 isolates were assumed to be non-tuberculosis mycobacteria (NTM)
  2. WT Wildtype, MUT Mutation