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Table 3 Mycobacterial strain identification using Hain Genotype line probe assay (LPA)

From: Bacteriological and molecular characterization of Mycobacterium bovis isolates from tuberculous lesions collected among slaughtered cattle, Northwest Ethiopia

Isolate nr.

Line probe assay (LPA) result

Mycobacterial identification

MTBc (TUB)

rpoB WT

rpoB MUT

katG WT

katG MUT

inhA WT

inhA MUT

H37Rv

+

+

–

+

–

+

–

M. tuberculosis

Distilled water

–

–

–

–

–

–

–

None

07

–

–

–

–

–

–

–

non-MTBc

12

+

+

–

+

–

+

–

MTBc

13

–

–

–

–

–

–

–

non-MTBc

16

+

+

–

+

–

+

–

MTBc

20

–

–

–

–

–

–

–

non-MTBc

21

+

+

–

+

–

+

–

MTBc

23

–

–

–

–

–

–

–

non-MTBc

36

+

+

–

+

–

+

–

MTBc

37

–

–

–

–

–

–

–

non-MTBc

38

–

–

–

–

–

–

–

non-MTBc

41

–

–

–

–

–

–

–

non-MTBc

43

–

–

–

–

–

–

–

non-MTBc

45

–

–

–

–

–

–

–

non-MTBc

48

+

+

–

+

–

+

–

MTBc

50

–

–

–

–

–

–

–

non-MTBc

54

–

–

–

–

–

–

–

non-MTBc

60

–

–

–

–

–

–

–

non-MTBc

61

–

–

–

–

–

–

–

non-MTBc

  1. The Hain Genotype LPA result from 18 culture-positive isolates obtained from LJ culture; five isolates (Isolate number: 12, 16, 21, 36, and 48) were confirmed as members of the M. tuberculosis complex (MTBc), while the remained 13 isolates were assumed to be non-tuberculosis mycobacteria (NTM)
  2. WT Wildtype, MUT Mutation