Transcriptional analysis reveals specific niche factors and response to environmental stresses of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents

Table 1 SCFA concentration and pH (± SEM) in filtered bovine digestive contents and M9-Glc before incubation (t=0) and after 3h and 6h incubation of EHEC EDL933

		Concentration (mM)
SCFA		T= 0	T= 3 h	T= 6h
acetate	M9-Glc Rumen Small Intestine Rectum	0.59 (± 0.03)^a 92.50 (± 0.66)^a 13.23 (± 0.20)^a 11.15 (± 0.17)^a	1.75 (± 0.10)^a - 15.40 (± 1.81)^a 12.08 (± 0.52)^a	5.42 (± 0.28)^b 83.65 (± 0.56)^b 21.12 (± 0.79)^b 11.81 (± 0.30)^a
propionate	M9-Glc Rumen Small Intestine Rectum	1.30 (± 0.05)^a 18.91 (± 0.18)^a 0.77 (± 0.09)^a 2.25 (± 0.17)^a	1.30 (± 0.07)^a - 0.63 (± 0.21)^a 2.23 (± 0.04)^a	1.26 (± 0.06)^a 16.92 (± 0.17)^a 4.85 (± 1.33)^b 2.10 (± 0.05)^a
butyrate	M9-Glc Rumen Small Intestine Rectum	0.60 (± 0.02)^a 12.48 (± 0.07)^a 0.17 (± 0.09)^a 0.82 (± 0.16)^a	0.66 (± 0.04)^a - 0.17 (± 0.03)^a 0.69 (± 0.04)^a	0.74 (± 0.05)^a 11.06 (± 0.13)^b 0.14 (± 0.02)^a 0.62 (± 0.03)^a
pH	M9-Glc Rumen Small Intestine Rectum	7.43 (± 0.01)^a 7.28 (± 0.18)^a 7.95 (± 0.02)^a 7.70 (± 0.07)^a	7.27 (± 0.03)^a - 7.74 (± 0.14)^a 7.40 (± 0.04)^a	6.86 (± 0.03)^b 7.13 (± 0.06)^a 7.27 (± 0.02)^b 7.48 (± 0.01)^a

^a,b: Within a row, means with a common letter are not statistically different (P < 0.05)
pH and SCFAs concentrations were measured in the incubation media (filtered rumen and small intestine contents were used without dilution, while the rectum content was diluted 1:1 with buffer, see the Materials and Methods section)

ISSN: 1471-2180