Skip to main content

Table 1 SCFA concentration and pH (± SEM) in filtered bovine digestive contents and M9-Glc before incubation (t=0) and after 3h and 6h incubation of EHEC EDL933

From: Transcriptional analysis reveals specific niche factors and response to environmental stresses of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents

   Concentration (mM)
SCFA   T= 0 T= 3 h T= 6h
acetate M9-Glc
Rumen
Small Intestine
Rectum
0.59 (± 0.03)a
92.50 (± 0.66)a
13.23 (± 0.20)a
11.15 (± 0.17)a
1.75 (± 0.10)a
-
15.40 (± 1.81)a
12.08 (± 0.52)a
5.42 (± 0.28)b
83.65 (± 0.56)b
21.12 (± 0.79)b
11.81 (± 0.30)a
propionate M9-Glc
Rumen
Small Intestine
Rectum
1.30 (± 0.05)a
18.91 (± 0.18)a
0.77 (± 0.09)a
2.25 (± 0.17)a
1.30 (± 0.07)a
-
0.63 (± 0.21)a
2.23 (± 0.04)a
1.26 (± 0.06)a
16.92 (± 0.17)a
4.85 (± 1.33)b
2.10 (± 0.05)a
butyrate M9-Glc
Rumen
Small Intestine
Rectum
0.60 (± 0.02)a
12.48 (± 0.07)a
0.17 (± 0.09)a
0.82 (± 0.16)a
0.66 (± 0.04)a
-
0.17 (± 0.03)a
0.69 (± 0.04)a
0.74 (± 0.05)a
11.06 (± 0.13)b
0.14 (± 0.02)a
0.62 (± 0.03)a
pH M9-Glc
Rumen
Small Intestine
Rectum
7.43 (± 0.01)a
7.28 (± 0.18)a
7.95 (± 0.02)a
7.70 (± 0.07)a
7.27 (± 0.03)a
-
7.74 (± 0.14)a
7.40 (± 0.04)a
6.86 (± 0.03)b
7.13 (± 0.06)a
7.27 (± 0.02)b
7.48 (± 0.01)a
  1. a,b: Within a row, means with a common letter are not statistically different (P < 0.05)
  2. pH and SCFAs concentrations were measured in the incubation media (filtered rumen and small intestine contents were used without dilution, while the rectum content was diluted 1:1 with buffer, see the Materials and Methods section)