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Table 4 The details of primers for the IS6110 and mpb64 genes

From: Two target genes based multiple cross displacement amplification combined with a lateral flow biosensor for the detection of Mycobacterium tuberculosis complex

Genes

Primers name a

Sequences and modifications

Length

IS6110

IS6110-F1

5′-GGATGGTCGCAGAGATCC-3′

18 nt

IS6110-F2

5′-ATCGCGTTCGCCCTT-3′

15 nt

IS6110-CP1

5′-CGCGCAGCCAACACCAAGTAGCAGCACGATTCGGAGTG-3′

38 mer

IS6110-CP2

5′-CCGGGACCACGACCGAAGACGCAATTCGGCGTTGTC-3’

36 mer

IS6110-C1*

5′-FITC-CGCGCAGCCAACACCAAGTAG-3’

21 nt

IS6110-C2

5′-CCGGGACCACGACCGAAGA-3’

19 nt

IS6110-D1

5′-ACCTCACTGATCGCTG-3’

16 nt

IS6110-D2

5′-ATCCGCTGAGCTGAAGC-3’

17 nt

IS6110-R1

5′-ACTTACGCACCGTCTC-3’

16 nt

IS6110-R2

5′-CAGGCGCAGGTCGATG-3’

16 nt

mpb64

mpb64-F1

5′-CCCCGGGTTGAAGAAGA-3’

17 nt

mpb64-F2

5′-GCTCAAGGTCTACCAGAAC-3’

19 nt

mpb64-CP1

5′-ACAGGTATCGATAGCGCCGAATGCCCCGTCGTTCGTGACT-3’

40 mer

mpb64-CP2

5′-TGCCACAGCGTGTCATAGGTACGACCACGTACAAGGC-3’

37 mer

mpb64-C1*

5′-Dig-ACAGGTATCGATAGCGCCGAATG-3’

23 nt

mpb64-C2

5′-TGCCACAGCGTGTCATAGGT-3’

20 nt

mpb64-D1

5′-CGGTGAATTATCAGAACTTC-3’

20 nt

mpb64-D2

5′-GCCTGGTCCCAATCGAA-3’

17 nt

mpb64-R1

5′-GTGAACTGAGCAAGCAGA-3’

18 nt

mpb64-R2

5′-ACAATGGGGAAGACGACT-3’

18 nt

  1. Abbreviations: FITC fluorescein isothiocyanate, Dig digoxigenin, mer monomeric unit, nt nucleotide
  2. a IS6110-C1*, 5′-labeled with FITC when used in the MCDA-LFB assay; mpb64-C1*, 5′-labeled with Dig when used in the MCDA-LFB assay