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Table 1 Reaction temperature optimization for MTBC-MCDA primers

From: Two target genes based multiple cross displacement amplification combined with a lateral flow biosensor for the detection of Mycobacterium tuberculosis complex

Temperature (°C)

IS6110 gene

mpb64 gene

Peak of turbidity

Time (min)

Peak of turbidity

Time (min)

63

0.16

34

0.18

29

64

0.14

31

0.20

27

65

0.17

32

0.21

26

66

0.18

30

0.21

25

67

0.18

30

0.22

23

68

0.16

34

0.21

25

69

0.15

38

0.20

25

70

0.13

55

0.20

26

  1. The threshold of turbidity> 0.1 was judged as positive for the MCDA reaction by a real-time turbidimeter
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BMC Microbiology

ISSN: 1471-2180

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