Fig. 4

Sensitivity of the MCDA method using serially diluted genomic DNA extracted from M. tuberculosis (H37Rv, ATCC27294). A total of two detection techniques, including the lateral flow biosensor (LFB) and colorimetric indicator (MG) methods, were applied to analyze the amplification products. Serial dilutions of target templates were subjected to standard MCDA reactions. DNA levels of 100 ng, 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg and 1 fg per reaction. Double distilled water was the template for the blank control. A Singly, the IS6110 gene at genomic DNA levels of 100 ng per reaction, 10 ng per reaction, 1 ng per reaction, 100 pg per reaction, 10 pg per reaction, 1 pg per reaction, 100 fg per reaction and 10 fg per reaction produced positive reactions. B The mpb64 gene at genomic DNA levels of 100 ng per reaction, 10 ng per reaction, 1 ng per reaction, 100 pg per reaction, 10 pg per reaction, 1 pg per reaction, 100 fg per reaction and 10 fg per reaction produced positive reactions. C Both the IS6110 gene and mpb64 gene at genomic DNA levels of 100 ng per reaction, 10 ng per reaction, 1 ng per reaction, 100 pg per reaction, 10 pg per reaction, 1 pg per reaction and 100 fg per reaction produced positive reactions