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Table 1 Fecal counts of gut microbiota as determined by RT-qPCR

From: Stool preparation under anaerobic conditions contributes to retention of obligate anaerobes: potential improvement for fecal microbiota transplantation

 

0H samples

 

1H samples

 
 

Anaerobic samples

Aerobic samples

P

Anaerobic samples

Aerobic samples

P

 

(n = 16)

(n = 16)

 

(n = 16)

(n = 16)

 

Total bacterial counts

10.1 ± 0.3

10.0 ± 0.3

 

10.2 ± 0.3

10.1 ± 0.3

 

Obligate anaerobe

      

Clostridium coccoides group

9.4 ± 0.4

9.2 ± 0.4

 

9.4 ± 0.4

9.2 ± 0.4

**0.0042

C.leptum subgroup

9.6 ± 0.4

9.5 ± 0.4

 

9.6 ± 0.4

9.5 ± 0.4

**0.1750

Bacteroides fragilis group

8.8 ± 0.6

8.8 ± 0.6

 

8.8 ± 0.6

8.6 ± 0.6

 

Bifidobacterium

8.9 ± 0.7

8.8 ± 0.8

 

9.0 ± 0.7

8.9 ± 0.7

 

Atopobium cluster

9.1 ± 0.5

9.0 ± 0.4

 

9.1 ± 0.5

9.0 ± 0.5

 

Prevotella

5.9 ± 1.9

6.1 ± 1.4

 

5.9 ± 1.9

5.6 ± 2.0

 

C.difficile

1.2 ± 0.0

1.3 ± 0.4

 

1.4 ± 0.9

1.2 ± 0.0

 

C.Perfringens

1.5 ± 1.0

1.4 ± 0.8

 

1.2 ± 0.0

1.8 ± 1.3

 

Faculative anaerobe

      

Total lactobacilli

5.8 ± 1.0

5.7 ± 1.4

 

6.0 ± 1.2

5.6 ± 1.5

 

Lactobacillus (formerly L. gasseri subgroup)

4.2 ± 2.1

4.3 ± 2.0

 

4.4 ± 2.2

4.2 ± 2.1

 

Levilactobacillus brevis (formerly L. brevis)

1.8 ± 1.3

1.6 ± 1.2

 

1.6 ± 1.1

1.6 ± 1.2

 

Lacticaseibacillus (formerly L. casei subgroup)

2.7 ± 1.8

3.7 ± 1.7

 

3.4 ± 1.9

3.4 ± 1.9

 

Limosilactobacillus fermentum (formerly L. fermentum)

3.0 ± 1.7

3.2 ± 1.6

 

2.9 ± 1.6

3.0 ± 1.5

 

Fructilactobacillus fructivorans (formerly L. fructivorans)

1.2 ± 0.0

1.2 ± 0.0

 

1.2 ± 0.0

1.2 ± 0.0

 

Lactiplantibacillus (formerly L. plantarum subgroup)

3.4 ± 1.7

3.4 ± 1.7

 

3.8 ± 1.3

3.4 ± 1.7

 

Limosilactobacillus (except L. fermentum) (formerly L. reuteri subgroup)

3.4 ± 1.9

3.2 ± 1.8

*0.0415

3.2 ± 2.0

3.3 ± 1.9

 

Ligilactobacillus and Liquorilactobacillus (formerly L. ruminis subgroup)

2.5 ± 2.2

2.3 ± 2.0

 

2.5 ± 2.1

2.6 ± 2.1

 

Latilactobacillus (formerly L. sakei subgroup)

2.4 ± 1.9

2.4 ± 1.9

 

2.6 ± 2.0

2.4 ± 2.0

 

Enterobacteriaceae

6.5 ± 0.7

6.4 ± 0.6

 

6.5 ± 0.7

6.4 ± 0.6

 

Enterococcus

5.6 ± 2.1

5.7 ± 2.1

 

5.9 ± 1.8

5.7 ± 2.1

 

Streptococcus

8.7 ± 0.6

8.6 ± 0.6

 

8.6 ± 0.6

8.5 ± 0.6

 

Staphylococcus

5.1 ± 0.9

5.2 ± 0.7

 

4.9 ± 1.1

5.1 ± 0.7

 
  1. The results are displayed as the means ± SD (log10 cells/g of feces)
  2. * p < 0.05, ** p < 0.01