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Fig. 1 | BMC Microbiology

Fig. 1

From: Stool preparation under anaerobic conditions contributes to retention of obligate anaerobes: potential improvement for fecal microbiota transplantation

Fig. 1

Stool collection and anaerobic and aerobic preparation. Fecal samples from 16 healthy adults were transferred under anaerobic conditions using an airtight container and deoxidizer and aliquoted into two portions in an anaerobic glove box. Each aliquot then was diluted with normal saline and blended for 30 s, either in the anaerobic glove box (anaerobic-prep) or under room air (aerobic-prep). Fecal samples were collected before blending (Sraw), and then immediately (0 h) and 1 h after anaerobic-prep (SAn0 and SAn1, respectively), or at 0 and 1 h after aerobic-prep (SAe0 and SAe1, respectively)

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