Table 3 Primers used in this study
Name | Sequence (5′–3′) | Amplifified fragment |
|---|---|---|
Primers for construction of mutants | ||
 ∆arcA-S-F | GCTCTAGATTTTGGCTAAATCTGTGTGC | ∆arcA-S (334 bp) |
 ∆arcA-S-R | GGTCAATTGCGTGGGCCAACTGCTTGCGCCTTC | |
 ∆arcA-X-F | GAAGGCGCAAGCAGTTGGCCCACGCAATTGACC | ∆arcA-X (466 bp) |
 ∆arcA-X-R | GGGGTACCTTTGAGAAGGTGATGCCG | |
|  |  | ∆arcA-SX (800 bp) |
 arcA-F | ATGCAAACCCCGCACATT | arcA (717 bp) |
 arcA-R | TTACTCTTCCAGCTCGCCG | |
Primers for identification of plasmid | ||
 pRE112-U-F | CACTGTTCGTCCATTTCCG | pRE112-UD (567 bp) |
 pRE112-D-R | TTCGTCTCAGCCAATCCCT | |
|  |  | pRE112-U-arcA-D (1284 bp) |
 pBAD33-U-F | AACAAAGCGGGACCAAAG | pBAD33-UD (529 bp) |
 pBAD33-D-R | AGAGCGTTCACCGACAAA | |
|  |  | pBAD33-U-arcA-D (1246 bp) |
 pET28a-U-F | TAATACGACTCACTATAGGG | pET28a-UD (318 bp) |
 pET28a-D-R | GCTAGTTATTGCTCAGCGG | |
|  |  | pET28a-U-arcA-D (1035 bp) |
Primers for construction of complemented strain | ||
 pBAD33-arcA+-F | GGGGTACCATGCAAACCCCGCACATT | arcA+ (733 bp) |
 pBAD33-arcA+-R | GCTCTAGATTACTCTTCCAGCTCGCCG | |
Primers for protein cloning | ||
 pET28a-arcA+-F | CGGGATCCATGCAAACCCCGCACATT | arcA+ (733 bp) |
 pET28a-arcA+-R | CCCTCGAGTTACTCTTCCAGCTCGCCG | |
Primers for qRT-PCR | ||
 16S rRNA-F | GGCAGCAGTGGGGAATATTG | 275 bp |
 16S rRNA-R | AGTTGAGCTCGGGGATTTCA | |
 qRT-flaK-F | CTGGTGGAGCGGTTGGTTAT | 254 bp |
 qRT-flaK-R | GGACCTTCATGACCAGCACA | |
 qRT-rpoN-F | AAACGGTGGAAATGCACGAA | 158 bp |
 qRT-rpoN-R | GACTAATGCTCGAATGGCCG | |
 qRT-cheV-F | AGCGCACACAATTAGTCGGA | 210 bp |
 qRT-cheV-R | CAATCGCCAAGCTCATGTCG | |
Primers for EMSA | ||
 EMSA-flaK-F | ACGGAAAGTCTTGACACTGTG | 309 bp |
 EMSA-flaK-R | TTTATGGCAGCGACTATAGC | |
 EMSA-rpoN-F | GTCGCCGAGTAGAAATTGCC | 350 bp |
 EMSA-rpoN-R | CGTACTTCCCGGCTAAGC | |
 EMSA-cheV-F | GGTAATAGTTTGCCGTCCCG | 378 bp |
 EMSA-cheV-R | TAACGTGCTACTCCCAGGG | |