Fig. 6

Relative abundance of bacterial species in iron-supplemented mice after antibiotic exposure. A-B Relative abundance of the two bacterial species that were detected as significantly different in abundance by GAMLSS-BEZI and C-D validated by real-time PCR, in mice fed an iron excess diet compared to mice fed an iron-sufficient diet during the recovery phase. Differences were assessed by Welch t-test. Bars represent the standard error of the mean (SEM) (N = 9 per time point and group). E, F Spearman’s correlations between luminal iron concentration at T80 of (E) B. vulgatus and (F) P. excrementihominis. r represents the correlation coefficient and dotted lines represent confidence interval at 95%. (N = 18)