Table 1 The primers sequences and the products sizes of the studied genes used in this study
Target | Gene | Primer sequence (5′-3′) | Annealing Tm | Size of product (bp) | Reference |
|---|---|---|---|---|---|
16 s rRNA | 16 s rRNA | F: AACTCTGTTATTAGGGAAGAACA R: CCACCTTCCTCCGGTTTGTCACC | 59 C ° for 1 min | 756 | [12] |
nuc | nuc | F: GCGATTGATGGTGATACGGTT R: AGCCAAGCCTTGACGAACTAAAGC | 59 C ° for 1 min | 279 | [12] |
mecA | mecA | F: GTAGAAATGACTGAACGTCCGATAA R: CCAATTCCACATTGTTTCGGTCTAA | 58C ° for 1 min | 310 | [14] |
Toxin genes | etB | F: ACAAGCAAAAGAATACAGCG R: GTTTTTGGCTGCTTCTCTTG | 53C° for 45 | 226 | [4] |
etA | F: GCAGGTGTTGATTTAGCATT R: AGATGTCCCTATTTTTGCTG | 53C° for 45 | 93 | [4] | |
pvl | F: ATCATTAGGTAAAATGTCTGGACATGATCC R: GCATCAAGTGTATTGGATAGCAAAAGC | 57 C ° for 30 s | 433 | ||
tsst-1 | F: TTATCGTAAGCCCTTTGTTG R: TAAAGGTAGTTCTATTGGAGTAGG | 53C° for 45 | 398 | [4] |