Table 1 Cultivation conditions
From: Cultivation of common bacterial species and strains from human skin, oral, and gut microbiota
Skin cultivation conditions | |
|---|---|
Media | Conditions |
LB agar | Aerobic, Anaerobic, 37 °C |
R2A agar | Aerobic, Anaerobic, 37 °C |
TSA with 5% sheep blood | Aerobic, Anaerobic, 37 °C |
Brucella agar | Aerobic, Anaerobic, 37 °C |
BCYE agar | Aerobic, Anaerobic, 37 °C |
MacConkey agar | Aerobic, Anaerobic, 37 °C |
Oral cultivation conditions | |
Media | Conditions |
LB agar | Aerobic, Anaerobic, 37 °C |
R2A agar | Aerobic, Anaerobic, 37 °C |
TSA with 5% sheep blood | Aerobic, Anaerobic, 37 °C |
Chocolate agar | Aerobic, Anaerobic, 37 °C |
Selective Strep agar | Aerobic, Anaerobic, 37 °C |
Stool cultivation conditions | |
Culture device prior to plating onto TSA with 5% sheep blood | Conditions |
Direct plating onto GMM agar | Anaerobic, 37 °C |
TSB (3, 7, 14d) + sheep blood (9% final vol) | Aerobic, 28 °C |
Aerobic blood bottle (3, 7, 14d) + rumen fluid (9% final vol) | Aerobic, 37 °C |
Aerobic blood bottle (3, 7, 14d) + sheep blood (9% final vol) | Aerobic, 37 °C |
Aerobic blood bottle (3, 7, 14d); sample filtered at 5 μm | Aerobic, 37 °C |
BHI (3, 7, 14d) + vancomycin + colistin (10 μg/mL each) | Aerobic, 37 °C |
TSB (3, 7, 14d) | Aerobic, 37 °C |
Anaerobic Blood bottle (3, 7, 14d) + sheep blood (9% final vol) | Anaerobic, 37 °C |
Anaerobic Blood bottle (3, 7, 14d) + rumen fluid (9% final vol) | Anaerobic, 37 °C |
Anaerobic Blood bottle (3, 7, 14d); sample filtered at 5 μm | Anaerobic, 37 °C |
Anaerobic Blood bottle (3, 7, 14d); thermic shock (85 °C, 20 min) | Anaerobic, 37 °C |
BHI (3, 7, 14d) + vancomycin + colistin (10 μg/mL each) | Anaerobic, 37 °C |
TSB (3, 7, 14d) + sheep blood (9% final vol) | Anaerobic, 37 °C |