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Table 1 Cultivation conditions

From: Cultivation of common bacterial species and strains from human skin, oral, and gut microbiota

Skin cultivation conditions
Media Conditions
LB agar Aerobic, Anaerobic, 37 °C
R2A agar Aerobic, Anaerobic, 37 °C
TSA with 5% sheep blood Aerobic, Anaerobic, 37 °C
Brucella agar Aerobic, Anaerobic, 37 °C
BCYE agar Aerobic, Anaerobic, 37 °C
MacConkey agar Aerobic, Anaerobic, 37 °C
Oral cultivation conditions
Media Conditions
LB agar Aerobic, Anaerobic, 37 °C
R2A agar Aerobic, Anaerobic, 37 °C
TSA with 5% sheep blood Aerobic, Anaerobic, 37 °C
Chocolate agar Aerobic, Anaerobic, 37 °C
Selective Strep agar Aerobic, Anaerobic, 37 °C
Stool cultivation conditions
Culture device prior to plating onto TSA with 5% sheep blood Conditions
Direct plating onto GMM agar Anaerobic, 37 °C
TSB (3, 7, 14d) + sheep blood (9% final vol) Aerobic, 28 °C
Aerobic blood bottle (3, 7, 14d) + rumen fluid (9% final vol) Aerobic, 37 °C
Aerobic blood bottle (3, 7, 14d) + sheep blood (9% final vol) Aerobic, 37 °C
Aerobic blood bottle (3, 7, 14d); sample filtered at 5 μm Aerobic, 37 °C
BHI (3, 7, 14d) + vancomycin + colistin (10 μg/mL each) Aerobic, 37 °C
TSB (3, 7, 14d) Aerobic, 37 °C
Anaerobic Blood bottle (3, 7, 14d) + sheep blood (9% final vol) Anaerobic, 37 °C
Anaerobic Blood bottle (3, 7, 14d) + rumen fluid (9% final vol) Anaerobic, 37 °C
Anaerobic Blood bottle (3, 7, 14d); sample filtered at 5 μm Anaerobic, 37 °C
Anaerobic Blood bottle (3, 7, 14d); thermic shock (85 °C, 20 min) Anaerobic, 37 °C
BHI (3, 7, 14d) + vancomycin + colistin (10 μg/mL each) Anaerobic, 37 °C
TSB (3, 7, 14d) + sheep blood (9% final vol) Anaerobic, 37 °C