Fig. 1
From: Cultivation of common bacterial species and strains from human skin, oral, and gut microbiota
Culturomics pipeline. A) Metagenomic data were generated for each oral (TD: tongue dorsum), skin (Fh: forehead), or stool sample. Example (non-representative) relative abundance plots of major species are shown with colors corresponding to phylum as shown in the legend. Samples were then B) diluted and cultivated in a defined set of anaerobic (green boxes) and aerobic (blue boxes) cultivation conditions for oral, skin, and stool. After a defined period of incubation, individual colonies were picked, subcultured for purity, then C) identified using MALDI-TOF, 16S rRNA gene sequencing, or whole genome sequencing