Strains | Description | Reference, Source, Sequence |
---|---|---|
M. huakii 7653R | Wild type, Nod+ on Astragalus sinicus | [45] |
HKabiEi | 7653R abiEi:pk19mob, Strr Neor | This study |
HKabiEi(pBBRabiEi) | Strain HKabiEi harboring plasmid pBBRabiEi | This study |
HKabiEi(pBBR1MCS-5) | Strain HKabiEi harboring plasmid pBBR1MCS-5 | This study |
Plasmids | ||
pETAbiE | AbiE_F/AbiE_R PCR product in pET-28a(+), Kmr | This study |
pETAbiEii | AbiE_F/AbiEii_R PCR product in pET-28a(+), Kmr | This study |
pK19mob | pK19mob pUC19 derivative lacZ mob Kmr | [46] |
pRK2013 | Helper plasmid for mobilizing plasmids Kmr | [47] |
pKabiEi | abiEiUP/abiEiLW PCR product in pK19mob, Kmr | This study |
pBBR1MCS-5 | lacPOZ′ mob, broad host range, Gmr | [48] |
pBBRabiEi | cabiEi_F/cabiEi_R PCR product in pBBR1MCS-5, Gmr | This study |
Primera | ||
AbiE_F | Sense primer used for AbiE system and abiEii gene expression | TTTGGATCCATGTCCTTGGTTGAACCCGA |
AbiE_R | Antisense primer used for AbiE system expression | TTTAAGCTTGTGCAGGTCGTAGTAGTGGC |
AbiEii_F | Antisense primer used for MCHK_RS33185 (abiEii) expression | TTTGGATCCGTGAGCACCGACGCCTATCG |
abiEiUP | Sense primer for MCHK_RS33180 (abiEi) mutation | TTTAAGCTTGAACCCGACAGCGATCTCCG |
abiEiLW | Antisense prime for MCHK_RS33180 (abiEi) mutation | TTTTCTAGAACTTGGCCTCGCTCGATGAG |
abiEipimap | Mapping PCR primer for abiEi | TGACTCCAGCGCAGCCGTCA |
cabiEi_F | Sense primer for abiEi complementation | TTTAAGCTTGCCACCGACCTTTTATCCTG |
cabiEi_R | Antisense primer for abiEi complementation | TTTTCTAGAGCCCTTGAACAGCAGCCGCG |
pK19A | pK19mob mapping primer | ATCAGATCTTGATCCCCTGC |
pK19B | pK19mob mapping primer | GCACGAGGGAGCTTCCAGGG |
Q16SrRNAF | Sense primer for qRT-PCR of 16S rDNA | AACTGAGATGGCTTTTGGAG |
Q16SrRNAR | Antisense primer for qRT-PCR of 16S rDNA | GGATGACGTCAAGTCCTCAT |
Q32450F | Sense primer for qRT-PCR of MCHK_RS32450 | TGGCGAGGTTACCGTACTCA |
Q32450R | Antisense primer for qRT-PCR of MCHK_RS32450 | AGCGTGTCTGAGCCGGCAAA |
Q32465F | Sense primer for qRT-PCR of MCHK_RS32465 | GCTCTACCAGAACCGCTATC |
Q32465R | Antisense primer for qRT-PCR of MCHK_RS32465 | TCGTACAACTCGTAGCATTT |
Q32345F | Sense primer for qRT-PCR of MCHK_RS32345 | CAACATCCATCAGATCACGA |
Q32345R | Antisense primer for qRT-PCR of MCHK_RS32345 | TGGCGCATTTCTCCGTCTTT |
Q31505F | Sense primer for qRT-PCR of MCHK_RS31505 | CAATGGAGTTTCTCAATGGA |
Q31505R | Antisense primer for qRT-PCR of MCHK_RS31505 | TATAGAGAAGCAGAGGCGTT |
Q31555F | Sense primer for qRT-PCR of MCHK_RS31555 | TGATCAGGCAGAACCACGGC |
Q31555R | Antisense primer for qRT-PCR of MCHK_RS31555 | ATCAAGGTCCGTACCCGTTA |
Q31330F | Sense primer for qRT-PCR of MCHK_RS31330 | TCGGCTCAGAATATCCTCGT |
Q31330R | Antisense primer for qRT-PCR of MCHK_RS31330 | CGAAATGAAGTCGCTTAGTA |
Q31340F | Sense primer for qRT-PCR of MCHK_RS31340 | ATTGCCAGCGCCAGCCAGGG |
Q31340R | Antisense primer for qRT-PCR of MCHK_RS31340 | GGCCGGGCTGCTCTATCTCG |
QabiEiF | Sense primer for qRT-PCR of abiEi | TCAAGAGCTTCGCCGGATCG |
QabiEiR | Antisense primer for qRT-PCR of abiEi | ACGGATCACGGCGCGATAGT |
QabiEiiF | Sense primer for qRT-PCR of abiEii | GGCCCGCTCAATGAGTTCCT |
QabiEiiR | Antisense primer for qRT-PCR of abiEi | TCTGATACCAAAGCAGCAGG |