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Table 1 Characteristics of isolates recoverd from the same patients before (A) and after (B) ceftazidime/avibactam exposure

From: Increased gene expression and copy number of mutated blaKPC lead to high-level ceftazidime/avibactam resistance in Klebsiella pneumoniae

     MIC Porin sequcence modifications
    KPN clinical isolate E.coli DH5a(clone)   
Strain KPCa β-lactamase CZAa CZA + 25 mg/ml PABN CZAa Ompk35b Ompk36c
1A KPC-2 TEM,SHV-64,CTX-M-65 2 d   truncated at 62 aa GD insertion at 136–137 aa
3A KPC-2 TEM,SHV-11,CTX-M-65 4    truncated at 62 aa GD insertion at 136–137 aa
7A KPC-2 SHV-64,OXA-10,DHA-1 4    truncated at 62 aa GD insertion at 136–137 aa
8A KPC-2 SHV-64,DHA-1 4 4 ≤0.125 truncated at 62 aa GD insertion at 136–137 aa
1B KPC-51 TEM,SHV-64,CTX-M-65 2048 2048 8 truncated at 62 aa GD insertion at 136–137 aa
3B KPC-33,KPC-2 TEM,SHV-11,CTX-M-65 256 512 2 truncated at 62 aa GD insertion at 136–137 aa
7B KPC-52 SHV-64,OXA-10,DHA-1 256 512 32 truncated at 62 aa GD insertion at 136–137 aa
8B KPC-33 SHV-64,DHA-1 128 128 2 truncated at 62 aa GD insertion at 136–137 aa
  1. Notes
  2. a Data were from the reported paper [25].
  3. b Predicted translational modification of Ompk35 were based on the reference sequence (NCBI reference sequence WP_135730820.1) from K. pneumoniae ATCC 13883.
  4. c Predicted translational modification of Ompk36 were based on the reference sequence (GenBank accession number AEW62399.1) from K. pneumoniae ATCC 13883.
  5. d The MIC of CZA + 25 mg/mL PABN were not detected in isolates 1A,3A, and 7A.
  6. Abbreviations: CZA ceftazidime/avibactam