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Table 1 List of oligonucleotide sequences and cycling conditions that used in this study

From: Emerging MDR-Mycobacterium avium subsp. avium in house-reared domestic birds as the first report in Egypt

Target genes Oligonucleotide sequences (5′-3′) Product size (bp) Initial
D
Cycling (35) Final E
D A E
M. avium
IS901
(F) 5′-GGATTGCTAACCACGTGGTG-3′ 577 94 °C
5 min.
94 °C
30 s.
58 °C
40 s.
72 °C
45 s.
72 °C
10 min.
(R) 5′- GCGAGTTGCTTGATGAGCG-3′
rpoB (F) 5′- TCAACATCCGTCCCGTCG-3′ 347 94 °C
5 min.
94 °C
30 s.
60 °C
40 s.
72 °C
45 s.
72 °C
10 min.
(R) 5′-GGCGGTCAGGTAGTGGAT-3′
rpsL (F) 5′-ACCAGTTGCGACCCGTAGA-3′ 592 94 °C
5 min.
94 °C
30 s.
56 °C
40 s.
72 °C
45 s.
72 °C
10 min.
(R) 5′-CGCCTAACCGTAAGGAAGTGAA-3′
otrB (F) 5′-CCGACATCTACGGGCGCAAGC-3′ 947 94 °C
5 min.
94 °C
30 s.
68 °C
40 s.
72 °C
1 min.
72 °C
10 min.
(R) 5′-GGTGATGACGGTCTGGGACAG-3′
inhA (F) 5′ - TGGTCAGCTTCCTGGCTTCC-3′ 810 94 °C
4 min
95 °C
1 min
55 °C
2 min
72 °C
2 min
72 °C
5 min
(R) 5′ - GACCGTCATCCAGTTGTAG-3′
  1. D Denaturation, A Annealing, E Extension