Table 4 Oligonucleotide primers used in multiplex PCR for gene based resistance testing
Primer | Target | Primer sequence 5′ – 3’ | Size |
|---|---|---|---|
tetmS – F | tet(M) | GTCTTGCATATATACGCCTTTATAGTGGAGTACTACATTTACGAG | 374 bp |
tetmA – R | tet(M) | CCACGTAATATCGTAGAAGCGGATCACTATCTGAG | |
tetoS – F | tet(O) | CGTATATATAGCGGAACATTGCATTTGAGGG | 548 bp |
tetoA – R | tet(O) | CGGCTCTATGGACAACCCGACAGAAG |