A multi-country study using MALDI-TOF mass spectrometry for rapid identification of Burkholderia pseudomallei

BMC Microbiology

Table 1 Identification accuracy of B. pseudomallei SuperSpectra by site

SuperSpectra	Spectrum acquisition site/isolate origin	Correctly identified spectra (%)	Correctly identified isolates (%)	Unidentified spectra (%)	Unidentified isolates (%)	Misidentified spectra (%)	Misidentified isolates (%)
SMRU^a	SMRU	15/15 (100)	5/5 (100)	0	0	0	0
	COMRU	90/90 (100)	30/30 (100)	0	0	0	0
	LOMWRU	41/57 (71.9)	18/19 (94.7)	16/57 (28.1)	1/19 (5.3)	0	0
	Townsville [18]^c,d	143/715 (20.0%)	36/76 (47.4)	563/715 (78.7)	33/76 (43.4)	9/715 (1.3)	7/76 (9.2)
	Asia	148/192 (77.1)	54/55 (98.2)	44/182 (22.9)	1/54 (1.9)	0	0
	Australia	136/685 (19.9)	35/75 (46.7)	540/685 (78.8)	33/75 (44.0)	9/685 (1.3)	7/75 (9.3)
SMRU+Townsville^b	SMRU	14/15 (93.3)	5/5 (100)	1/15 (6.7)	0	0	0
	COMRU	277/285 (97.2)	95/95 (100)	8/285 (2.8)	0	0	0
	LOMWRU	38/57 (66.7)	19/19 (100)	19/57 (33.3)	0	0	0
	Townsville/Australia^e	59/62 (95.2)	31/31 (100)	2/62 (3.2)	0	1/62 (1.6)^f	0
	Asia^e	329/357 (92.2)	119/119 (100)	28/357 (7.8)	0	0	0

^aSMRU-SS-Bps1 and SMRU-SS-Bth1
^bSMRU-SS-Bps1-3, SMRU-SS-Bth1, Townsville-SS-Bps128, Townsville-SS-Bps347, Townsville-SS-Bps457, Townsville-SS-Bps694, Townsville-SS-Bps854, Townsville-SS-BpsATCC4846, and Townsville-SS-BpsATCC23343
^cSeventy-four isolates from Australia, 1 isolate from Asia and 1 isolate of unknown origin
^dAvailable existing spectra from the Townsville study were used and bacterial isolates were not re-cultured
^eIndependent isolates that were not used in the SuperSpectra creations
^fMisidentified as Sphingobacterium multivorum (91.5% ID) another spot of the same isolate was correctly identified as B. pseudomallei (99.9% ID)

ISSN: 1471-2180