Fig. 5

Determination of reactive oxygen species levels and nitrite production after treatment with AgNP-PVP-MA nanocomposite (AgNP to 1 %, 0.1 g of PVP and 50 µg/mL of MA). Generation of ROS in macrophages infected with Leishmania amazonensis and treated with nanocomposite for 24 h (A). Levels of nitrite production in macrophages infected with Leishmania amazonensis and treated with nanocomposite for 24 h (B). Control group: macrophages infected and untreated. AgNP-PVP-MA group: macrophages infected and treated with the nanocomposite. AMB group: macrophages infected and treated with amphotericin B at a concentration of 0.5 µg/mL. The results were expressed as mean ± standard deviation of triplicate sample (n = 3) in three different experiments with analysis of variance, Student’s t-test; *p ≤ 0.05