Bacteria, plasmid, oligonucleotides | Relevant characteristic(s) or sequence | Source | |
---|---|---|---|
Bacterial strains | |||
 E. coli DH5αλpir | Plasmid cloning strain | [21] | |
 S. aureus RN4220 | Restriction deficient strain, partially defective AgrA | [43] | |
 S. aureus 10400326 or Sa2 | ST351, AIP-II producer | [44] | |
 S. aureus 10303344 or Sa25 | ST352, AIP-I producer | [44] | |
 S. aureus 41000044 or Sa27 | ST151, AIP-II producer | [44] | |
 S. aureus 21000024 or Sa30 | ST352, AIP-I producer | [44] | |
 L. lactis subsp. lactis ATCC 11454 | Nisin producer | [45] | |
 S. epidermidis ATCC 14990 | AIPep-I producer | [46] | |
Plasmids | |||
 pBGR1 | Promoter trap vector | [47] | |
 pKK30 | Highly stable plasmid containing PsarAP1-dfrA | [21] | |
 pQS1 | pKK30 containing PagrP3-gfpbk | This study | |
 pQS3 | pKK30 containing PagrP3-mCherry | This study | |
Primers | |||
 AGRP3-F3 (EcoRI) | AAAGAATTCGTAATTTGTATTTAATATTTTAAC | This study | |
 AGRP3-F4 (NheI) | AAAGCTAGCGTAATTTGTATTTAATATTTTAAC | This study | |
 AGRP3-R1 (BamHI) | AAAGGATCCCAACTATTTTCCATCAC | This study | |
 GFP-R1 (EcoRV) | AAAGATATCTTATTTGTAGAGCTC | This study | |
 mCherry-R1 (BamHI) | AAAGGATCCCTACTTGTACAGCTC | This study |